The bacterial rows
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The rows
Coliform total and fecal and E. coli, fecal streptococci, enterococci and Salmonella: container PA or G, cool to below 10 degrees C with 0.008 percent sodium thiosulfate, 8 hours.
PA is a property, not a resin
"Any plastic that is made of a sterilizable material (polypropylene or other autoclavable plastic)." The container has to be sterile, which is the whole point for a bacterial count.
Which our catalog cannot confirm
We record material and not autoclavability. 13 polypropylene containers here, which is the resin the footnote names as an example, with no sterilization claim attached.
So for bacterial work, buy sterile
Pre-sterilised sample containers are a product with a documented process behind them, and they are not what a packaging catalog sells. Said plainly rather than left to be discovered.
The thiosulfate is in the container before you arrive
0.008 percent sodium thiosulfate neutralises residual chlorine so the sample stops being disinfected on the way to the laboratory. It is normally supplied in the bottle.
Which is another reason the bottle is bought, not borrowed
A container that arrives with a measured reagent in it is a prepared object. Rinsing it, or filling it to overflowing, removes the reagent and the sample is no longer preserved.
Do not rinse a bacterial bottle
The instinct from every other kind of sampling is to rinse the container with the water being sampled. Here it throws away the thiosulfate and risks the sterility.
Below 10 degrees, not below 6
The bacterial rows differ from most of the table, which says 6 or below. Worth noticing rather than applying one number to everything in the cooler.
And leave a headspace
Unlike the volatile parameters, a bacterial sample is usually filled with room to mix. The laboratory shakes it before plating.
Eight hours decides where you can sample
Collection, transport and the start of analysis within one working day. A remote site, a Friday afternoon or a laboratory two hours away can make a bacterial sample impossible rather than merely difficult.