It was not sterile
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A clean container was treated as sterile
Washed, dried and capped is clean. Sterile is a validated process with a record, and for culture work the difference appears as a colony that should not be there.
Which contaminates the result, not the container
A plate with an unexpected organism is a question nobody can answer afterwards: was it the sample, the media, the bottle or the bench.
HDPE was autoclaved
Polyethylene is not the material chosen for autoclave duty. Polypropylene is, and glass is, and there are 13 polypropylene and 103 glass containers here against 154 HDPE.
We cannot tell you which of ours survive it
Our catalog records material, capacity and neck finish, not autoclavability. That is a question for the manufacturer with the bottle, closure and liner all named.
The cap was tightened for the cycle
A sealed container in an autoclave is a pressure vessel. Caps go on loosely for the cycle and are tightened after, and the one that was tightened first deforms or bursts.
Thermal shock cracked the glass
Molten agar into a cold bottle, or a hot bottle onto a wet bench. Media work cycles between autoclave temperature and a water bath, repeatedly, in the same vessel.
The liner degraded and nobody looked
Cycled to sterilisation temperature many times, a liner hardens, cracks and stops sealing. The bottle looks perfect and the media dries out over a month.
The media had no date on it
Which is the quiet one. A bottle of clear medium with no made-on date gets used, and the experiment it ruins is not traced back to it.
A reagent bottle was reused for a specimen
Different regime, different requirements, and nothing about a reagent bottle makes it a specimen container. The four leaves we do not make are the ones this happens to.
Buy the right thing for the sterile step
Pre-sterilised where sterility is the requirement, autoclavable where you will sterilise it yourself and the manufacturer says so, and our bottles for everything that is neither.